Device

Part:BBa_K4175008:Design

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-08-30)


IL-6_scfv-Notch-Gal4KRAB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BglII site found at 1052
    Illegal BamHI site found at 76
    Illegal XhoI site found at 19
    Illegal XhoI site found at 1036
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1144
    Illegal PstI site found at 1375
    Illegal PstI site found at 1416
    Illegal PstI site found at 1462
    Illegal PstI site found at 2494
    Illegal NgoMIV site found at 1288
    Illegal NgoMIV site found at 2279
    Illegal NgoMIV site found at 2414
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None.


Source

anti-IL-6 scFv (BBa_K4175000), Notch core (BBa_K4175001), ZF_GAl4_KRAB (BBa_K2446037).

References