Device
Part:BBa_K4175008:Design
Designed by: Li Xianxiu, Zhang Wanying Group: iGEM22_ZJUintl-China (2022-08-30)
IL-6_scfv-Notch-Gal4KRAB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55
Illegal PstI site found at 1144
Illegal PstI site found at 1375
Illegal PstI site found at 1416
Illegal PstI site found at 1462
Illegal PstI site found at 2494 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 60
Illegal PstI site found at 55
Illegal PstI site found at 1144
Illegal PstI site found at 1375
Illegal PstI site found at 1416
Illegal PstI site found at 1462
Illegal PstI site found at 2494 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 60
Illegal BglII site found at 1052
Illegal BamHI site found at 76
Illegal XhoI site found at 19
Illegal XhoI site found at 1036 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55
Illegal PstI site found at 1144
Illegal PstI site found at 1375
Illegal PstI site found at 1416
Illegal PstI site found at 1462
Illegal PstI site found at 2494 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 60
Illegal XbaI site found at 271
Illegal PstI site found at 55
Illegal PstI site found at 1144
Illegal PstI site found at 1375
Illegal PstI site found at 1416
Illegal PstI site found at 1462
Illegal PstI site found at 2494
Illegal NgoMIV site found at 1288
Illegal NgoMIV site found at 2279
Illegal NgoMIV site found at 2414
Illegal AgeI site found at 1096 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
None.
Source
anti-IL-6 scFv (BBa_K4175000), Notch core (BBa_K4175001), ZF_GAl4_KRAB (BBa_K2446037).